UCLA-DOE Biosciences

If the data was acquired with TS3 (native)

Just process as you would any TopSpin dataset:     xfb     ftnd 0     ftnd 0 dlp

If the data was acquired with hmsIST/TS3

As above. Just process as you would any TopSpin dataset.

If the data was acquired with TS2 (or TS1 or xwinnmr)

This type of data can also be processed in TS3, but you have to change some parameters.

As an example, consider an hncacb which is Echo-Antiecho in F2 (15N) and States-TPPI in F1 (13C),

run with 64 nominal (complex) points in F1 and 55 nominal (complex) points in F2.

With ~30% sampling, there are 1058 points in the sampling schedule (file: sched).

When the experiment was run, the TD values were:  F3:2048   F2:4   F1:1058

To process this dataset in TS3, first open the dataset in TS3.

Set “s td” to:   F3:2048   F2:4232   F1:1                       (4232 = 1058*4)

Set “NUSTD” and “s NUSTD” to:    F3:2048   F2:110   F1:128                       (110=2*55, 128=2*64)

Check “s FNmode”, and make sure that the values are: F2:Echo-Antiecho   F1:States-TPPI

In a linux shell, go into the dataset directory. Copy the file “sched” to the file “nuslist”  (ie: >cp sched nuslist)

Then process normally  (eg ftnd 0)

 

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